Opsonization of merozoites with sera from Theileria sergenti-infected calves.

Expression of Recombinant Streptokinase from Streptococcus Pyogenes and Its Reaction with Infected Human and Murine Sera

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...

NOTE Clinical Pathology Cytokine and Inducible Nitric Oxide Synthase Gene Expressions in Peripheral Blood Mononuclear Cells and Related Clinical Characteristics in Theileria orientalis sergenti-Infected Calves

Eight splenectomized calves were inoculated with Theileria orientalis sergenti (Tos)-infected tick gland homogenate (5 calves) or infected erythrocyte suspension (3 calves). Clinical characteristics were different in calves post-infection. Animals were divided into 3 groups on the basis of susceptibility as high, middle, and low. Increase in mRNA of IFNγ, IL-2, and TNF-α was observed in periphe...

Cloning and Expression of Immunogenic Regions of EMA-1 Gene of Theileria equi From Infected Horses

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...

Western Blot Analysis of Sera from Leishmania major-Infected BALB/c and C57BL/6 Mice

In Leishmania (L.) major-infected BALB/c mice Th2-type cells results in disease progression, whereas C57BL/6-infected mice mount a Th1-type response, which leads to control of the infection. Th2 response correlates with IgG1 whereas, Th1 response supports switching to IgG2a subclasses. Since IgG isotype-dominated response depends on different CD4 + T cell subsets, we studied the antigenic profi...

Unusual cases of Theileria annulata infection in calves